Breckwoldt, M. et al. Correlated magnetic resonance imaging and ultramicroscopy (MR-UM) is a tool kit to assess the dynamics of glioma angiogenesis. eLife, 2016; 5
Brain tumor vascularisation is visualized using T2*-weighted magnetic resonance imaging.
Credit: Breckwoldt, Bode et al.
Stopping the growth of blood vessels in tumors is a key target for glioblastoma therapies, and imaging methods are essential for initial diagnosis and monitoring the effects of treatments. A team of researchers has developed a combined magnetic resonance imaging (MRI) and ultramicroscopy ‘toolkit’ to study vessel growth in glioma models in more detail than previously possible.
In their study in mice, the team combined an MRI approach in vivo with ultramicroscopy of ex vivo whole brains cleared for imaging.
The technique is based on T2*-weighted (T2*-w) MRI images, one of the basic pulse sequences in MRI, with high resolution to allow for substantially more detail than conventional T2*-w imaging. Pre- and post-contrast MR scans were performed to define the growth of vessels during glioma development in two different glioma models.
The team further mapped the development of vessels by dual-colour ultramicroscopy of whole, cleared brains. Using fluorescent labelling of microvessels, they collected complementary 3D MR and ultramicroscopy data sets (dubbed the ‘MR-UM’), which could be compared side-by-side.
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